Chemiluminescent energy-transfer cassettes based on fluorescein and nile red.
نویسندگان
چکیده
منابع مشابه
Chemiluminescent Energy-Transfer Cassettes Based on Fluorescein and Nile Red We thank Prof. Robin M. Hochstrasser (University of Pennsylvania) for helpful discussions and the National Institutes of Health (GM72041) and The Robert[emsp14]A. Welch Foundation for providing financial support
The two most common ways to induce chemiluminescence in purely organic, nonbiological systems are to treat either oxalate esters or luminol derivatives with basic hydrogen peroxide. Both these types of mixtures emit light at relatively short wavelengths, which are not ideal for applications in biotechnology. Luminol, for instance, emits in the range 420–450 nm, depending on the solvent media. I...
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We have designed fluorescent "through-bond energy-transfer cassettes" that can harvest energy of a relatively short wavelength (e.g., 490 nm), and emit it at appreciably longer wavelengths without significant loss of intensity. Probes of this type could be particularly useful in biotechnology for multiplexing experiments in which several different outputs are to be observed from a single excita...
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A special, water-soluble, fluorescent probe 1 was designed. This consisted of a fluorescein-based component to harvest irradiation at 488 nm and a rhodamine-based part designed to emit it at a significantly longer wavelength. This cassette was used to label an illustrative protein called ACBP. Evidence was accumulated to support the assertion that ACBP-1 bound its native ligand with a binding c...
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عنوان ژورنال:
- Angewandte Chemie
دوره 46 10 شماره
صفحات -
تاریخ انتشار 2007